Optimized Formation of Detergent Micelles of β-Carotene and Retinal Production Using Recombinant Human β,β-Carotene 15,15'-Monooxygenase
نویسندگان
چکیده
منابع مشابه
β,β-carotene 15,15'-monooxygenase and its substrate β-carotene modulate migration and invasion in colorectal carcinoma cells.
BACKGROUND β,β-Carotene 15,15'-monooxygenase (BCMO1) converts β-carotene to retinaldehyde. Increased β-carotene consumption is linked to antitumor effects. Retinoic acid reduces the invasiveness in cancer, through inhibition of matrix metalloproteinases (MMPs). In our studies of a mouse model that develops intestinal tumors after low dietary folate, we found reduced BCMO1 expression in normal p...
متن کاملCharacterization of human β,β-carotene-15,15'-monooxygenase (BCMO1) as a soluble monomeric enzyme.
The formal first step in in vitamin A metabolism is the conversion of its natural precursor β,β-carotene (C40) to retinaldehyde (C20). This reaction is catalyzed by the enzyme β,β-carotene-15,15'-monooxygenase (BCMO1). BCMO1 has been cloned from several vertebrate species, including humans. However, knowledge about this protein's enzymatic and structural properties is scant. Here we expressed h...
متن کاملLipid and β-Carotene Production by Rhodosporidium diobovatum Cultured with Different Carbon to Nitrogen Ratios
Background: In food industry, carotenoids are used as food colorants conferring yellow to red color. This research was designed to study on lipid and β-carotene production by Rhodosporidium diobovatum cultured with different Carbon to Nitrogen (C/N) ratios. Methods: R. diobovatum was cultured in a medium containing 40 g/l glucose (as the carbon source) and different C/N ratios (20, 50, and 80)...
متن کاملAltered expression of both β-carotene 15,15' monooxygenase and lecithin:retinol acyltransferase in obese Zucker rats.
Retinol and its active derivative retinoic acid have an important role in development, reproduction, immunity, and cell proliferation/differentiation. Obesity and dyslipidemia are risk factors for cardiovascular disease that may affect hepatic homeostasis. It is unclear whether the expression of retinol-related proteins is affected and influences the retinol status in obesity and dyslipidemia. ...
متن کاملExpression of -Carotene 15,15 Monooxygenase in Retina and RPE-Choroid
RESULTS. By RT-PCR, -CM mRNA was detected at a low level in mouse and monkey retina and in the RPE-choroid of the monkey but not of the mouse. Conversely, -CM mRNA was expressed at a low level in both human and bovine RPEchoroid, but not in the retina of either. RPE primary cultured cells of the monkey also showed -CM mRNA expression, although the three human lines did not. In addition, of nine...
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ژورنال
عنوان ژورنال: Biotechnology Progress
سال: 2008
ISSN: 8756-7938
DOI: 10.1021/bp070239k